Multi-Photon Microscopy

When researchers from the University of Chicago called us with their requirements for a custom microscopy mirror, we worked with them to develop just the right coating design. Specifically, they needed high reflectivity in the near-infrared, broad bandwidth, low dispersion and high transmission in the visible spectrum. They also wanted components that were compact, durable and environmentally stable.
Off-the-shelf optics available from other sources proved unsuitable for their ultrafast system because of wavelength-dependent dispersive properties and undesirable phase shifts. Fortunately PPC was able to come up with a cost-efficient proposition to supply them the right mirrors for their experiment in a short lead time.
Muntjac skin fibroblast cells with actin filaments labeled by Alexa 488. Excitation at 1035 nm. Emission detected at 520 nm using custom PPC mirrors.
Photos courtesy of the University of Chicago Gordon Center for Integrative Science.
Custom Dichroic Mirror for Microscopy

